Microbial ID

Rapid & Accurate
Fatty Acid Analysis & Microbial Identification

Microbial Identification Inc (MIDI), located in Newark, Delaware, USA, has been developing rapid fatty acid-based identification solutions for agriculture, environmental and clinical laboratories since 1991.

MIDI manufactures two systems for fatty acid analysis and microbial identification:

The Sherlock® Microbial Identification System (MIS)

The Sherlock® Mycobacteria Identification System (MYCO-LCS)

In addition, we provide contract laboratory services for bacterial and fungal identification (DNA sequencing and MALDI-TOF), as well as Probiotic Enumeration, Fatty Acid Analysis (FAME) and
Soil Phospholipid Fatty Acid
Analysis (PLFA):


Microbial ID - FAME and PLFA

MIDI is a small, veteran-owned biotechnology business and a worldwide Premier Channel Partner of Agilent Technologies.


Sherlock® Microbial ID System (MIS)

Cellular fatty acid analysis by Gas Chromatography (FAME) has been used for more than 50 years as a rapid and
inexpensive microbial identification method.

Phospholipid fatty acid analysis (PLFA) has been used for more than 30 years to characterize and monitor changes in the soil microbiota.

The Sherlock® MIS is the
only commercially-available system that can be used for both microbial ID and PLFA.  There are more than 1,000 peer-reviewed publications in which the MIDI System was used, including more than 300 for PLFA and Soil Science.

The Sherlock® MIS can identify over 150 fatty acids and 1,500 bacterial species in as little as 15 minutes. For microbial ID, no Gram stain or biochemical cards are needed and consumables cost as little as $3 USD per test.

The Sherlock® MIS software includes many powerful tools, including: PLFA Analysis (PLFA Tools) Library Generation (LGS), Principal Component Analysis (PCA), and Electronic Records & Signatures (ERS).

For research use only.  Not for use in diagnostic procedures.

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Sherlock® Mycobacteria ID System (MYCO-LCS)

Cellular mycolic acid analysis by High Performance Liquid Chromatography (HPLC) has been used for more than 20 years as a rapid and easy-to-use method for routine mycobacterial ID.

The Sherlock® MYCO-LCS
is the only commercially-available mycobacterial ID system that uses mycolic
acid analysis by HPLC for
Mycobacterium tuberculosis ID.

The Sherlock® MYCO-LCS identifies over 25 species
of mycobacteria and 35 species of non-mycobacteria, including: Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella.

Sherlock® MYCO-LCS can separate M. bovis BCG from the TB complex and
M. scrofulaceum
from MAI.

The Sherlock® MYCO-LCS uses the same procedure to identify all mycobacterial and non-mycobacterial species in less than 30 minutes from pure culture.  The cost per test for consumables is less than $6 USD.

For research use only.  Not for use in diagnostic procedures.

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AOCS' Inform Article

The Sherlock software was featured in the October 2014 issue of Inform, the monthly publication of the American Oil Chemists' Society (AOCS).

Automated Analysis of Edible Oils

AOCS Inform Article

Upcoming MIDI Events

November 2-5, 2014

2015 PDA Annual
Annual Meeting

Red Rock Casino Resort & Spa
Las Vegas, NV USA

Booth # 306

event website


Soil & Edible Oil Analysis

The Sherlock software has been expanded to automate the analysis of edible oils with complex FAME patterns (e.g. fish oil, those with 18:1 isomers and cis/trans), soil PLFAs and Palm Tocotrienols (HPLC). Additional products that are in development include analysis of Triglycerides, Sterols and Triterpenes.

Fish Oil (Omega-3s)

Palm Oil (Tocotrienols)

Soils (PLFAs)

MIDI Product Brochure

A complete overview of MIDI's products and services.

MIDI Product Brochure

Please contact

for more information on our products and services.