Microbial ID

Automated Solutions to Complex Chromatographic Analysis Problems

MIDI, Inc., located in Newark, Delaware, USA, has been developing rapid fatty acid-based identification solutions for agriculture, environmental and clinical laboratories since 1991.

MIDI manufactures multiple automated chromatography solutions:

The Sherlock™ Microbial Identification System (GC)

  • PLFA Analysis
  • Microbial Identification
  • Edible Oil Analysis
The Sherlock™ Mycobacteria Identification System (HPLC)
  • Mycobacterial Identification
The Sherlock™ X Identification System (GC-MS)
  • Fire Debris Analysis

In addition, we provide contract laboratory services for bacterial and fungal identification (DNA sequencing and MALDI-TOF), as well as Probiotic Enumeration, Fatty Acid Analysis (FAME) and
Soil Phospholipid Fatty Acid
Analysis (PLFA):


Microbial ID - FAME and PLFA

MIDI is a small, veteran-owned biotechnology business and a worldwide Premier Channel Partner of Agilent Technologies.


Sherlock™ Microbial ID System (MIS)

Fatty acid (FAME) analysis by Gas Chromatography has been used for more than 50 years as a rapid microbial identification method.

Phospholipid fatty acid analysis (PLFA) has been used for more than 30 years to characterize and monitor broad changes in the "living" soil microbiota.

The Sherlock MIS is the
only commercially-available system that can be used for both microbial ID and PLFA.  There are more than 1,000 peer-reviewed publications referencing the MIDI Sherlock System, including more than 300 for PLFA and Soil Science.

The Sherlock MIS software includes many powerful tools: Peak Categorization, Library Generation (LGS), Principal Component Analysis (PCA), and Electronic Records & Signatures (ERS).

Since 2014, Sherlock's precision peak naming and categorization capabilities have been adopted to automate other complex chromatographic datasets. Examples include fire debris and edible oil analysis.

For research use only.  Not for use in diagnostic procedures.

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Sherlock™ Mycobacteria ID System (MYCO-LCS)

Cellular mycolic acid analysis by High Performance Liquid Chromatography (HPLC) has been used for more than 20 years as a rapid and easy-to-use method for routine mycobacterial ID.

The Sherlock™ MYCO-LCS
is the only commercially-available mycobacterial ID system that uses mycolic
acid analysis by HPLC for
Mycobacterium tuberculosis ID.

The Sherlock™ MYCO-LCS identifies over 25 species
of mycobacteria and 35 species of non-mycobacteria, including: Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella.

Sherlock™ MYCO-LCS can separate M. bovis BCG from the TB complex and
M. scrofulaceum
from MAI.

The Sherlock™ MYCO-LCS uses the same procedure to identify all mycobacterial and non-mycobacterial species in less than 30 minutes from pure culture.  The cost per test for consumables is less than $6 USD.

For research use only.  Not for use in diagnostic procedures.

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Upcoming MIDI Events

January 5-7, 2016

Northeastern Plant, Pest &
Soils Conference

Sheraton Society Hill
Philadelphia, PA, USA

Tabletop Display

Event Website


Soils, Oils & Forensics

The Sherlock software platform has expanded to solve a variety of complex chromatography problems, which include the forensics analysis of fire debris residues, edible oil authentication and soil PLFA analysis

Sherlock X: Fire Debris Analysis

Edible Oil & Fish Oil Analysis

Soil Microbial Community Analysis

MIDI Product Brochure

A complete overview of MIDI's products and services.

MIDI Product Brochure

Please contact

for more information on our products and services.