Microbial ID

Rapid & Accurate
Fatty Acid Analysis & Microbial Identification

Microbial Identification Inc (MIDI), located in Newark, Delaware, USA, has been developing rapid fatty acid-based identification solutions for agriculture, environmental and clinical laboratories since 1991.

MIDI manufactures two systems for fatty acid analysis and microbial identification:

The Sherlock® Microbial Identification System (MIS)

The Sherlock® Mycobacteria Identification System (MYCO-LCS)

In addition, we provide contract laboratory services for bacterial and fungal identification (DNA sequencing and MALDI-TOF), as well as Probiotic Enumeration, Fatty Acid Analysis (FAME) and
Soil Phospholipid Fatty Acid
Analysis (PLFA):


Microbial ID - FAME and PLFA

MIDI is a small, veteran-owned biotechnology business and a worldwide Premier Channel Partner of Agilent Technologies.

Sherlock® Microbial ID System (MIS)

Cellular fatty acid analysis by Gas Chromatography (FAME) has been used for more than 50 years as a rapid and
inexpensive microbial identification method.

The Sherlock® MIS is the
only commercially-available microbial ID system that uses FAME technology.  The system is U.S. CDC cleared for aerobic bacterial ID and
AOAC INTERNATIONAL cleared for Bacillus anthracis ID.

The Sherlock® MIS can identify over 1,500 bacterial and yeast species in as little as 15 minutes from pure culture, with no Gram stain or biochemical cards required.  Consumables cost as little as $3.00 USD per test.

The Sherlock® MIS software includes many powerful tools, including: custom library generation (LGS), principal component analysis (PCA), dendrogram, strain tracking and Electronic Records & Signatures (ERS).

Many Sherlock® MIS customers use the system to identify fatty acids (such as PLFAs), because of the precision of the system to name over 150 fatty acids and related compounds.

For research use only.  Not for use in diagnostic procedures.

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Sherlock® Mycobacteria ID System (MYCO-LCS)

Cellular mycolic acid analysis by High Performance Liquid Chromatography (HPLC) has been used for more than 15 years as a rapid and easy-to-use method for routine mycobacterial ID.

The Sherlock® MYCO-LCS
is the only commercially-available mycobacterial ID system that uses mycolic
acid analysis by HPLC for
Mycobacterium tuberculosis ID.

The Sherlock® MYCO-LCS identifies over 25 species
of mycobacteria and 35 species of non-mycobacteria, including: Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella.

Sherlock® MYCO-LCS can separate M. bovis BCG from the TB complex and
M. scrofulaceum
from MAI.

The Sherlock® MYCO-LCS uses the same procedure to identify all mycobacterial and non-mycobacterial species in less than 30 minutes from pure culture.  The cost per test is less than $6.00 USD
in consumables.

For research use only.  Not for use in diagnostic procedures.

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New MIDI Distributor - Brazil

Katálysis Instrumentação Científica is now MIDI's Exclusive Distributor in Brazil.  Katálysis

employee's have vast experience in support and maintenance of Agilent Technologies' equipment and the MIDI Sherlock System.

Katálysis Website

Phospholipid Fatty Acids (PLFA)

A new PLFA extraction protocol, fatty acid peak naming table and software tools were developed
to aid soil microbiologists and
soil scientists with microbial community research projects.

Rapid PLFA Extraction

Fatty Acids Identified

PLFA Analysis Tools

MIDI Product Brochure

A complete overview of MIDI's products and services.

MIDI Product Brochure

Please contact

for more information on our products and services.