Microbial ID

Rapid & Automated Chromatography Solutions

MIDI, Inc., located in Newark, Delaware, USA, has been developing rapid fatty acid-based identification solutions for agriculture, environmental and clinical laboratories since 1991.

MIDI manufactures multiple automated chromatography solutions:

The Sherlock™ Microbial Identification System (GC)

  • PLFA Analysis
  • Microbial Identification
  • Edible Oil Analysis
The Sherlock™ Mycobacteria Identification System (HPLC)
  • Mycobacterial Identification
The Sherlock™ X Identification System (GC-MS)
  • Fire Debris Analysis

In addition, we provide contract laboratory services for bacterial and fungal identification (DNA sequencing and MALDI-TOF), as well as Probiotic Enumeration, Fatty Acid Analysis (FAME) and
Soil Phospholipid Fatty Acid
Analysis (PLFA):


Microbial ID - FAME and PLFA

MIDI is a small, veteran-owned biotechnology business and a worldwide Premier Channel Partner of Agilent Technologies.


Sherlock™ Microbial ID System (MIS)

Cellular fatty acid analysis by Gas Chromatography (FAME) has been used for more than 50 years as a rapid and
inexpensive microbial identification method.

Phospholipid fatty acid analysis (PLFA) has been used for more than 30 years to characterize and monitor changes in the soil microbiota.

The Sherlock™ MIS is the
only commercially-available system that can be used for both microbial ID and PLFA.  There are more than 1,000 peer-reviewed publications in which the MIDI System was used, including more than 300 for PLFA and Soil Science.

The Sherlock™ MIS can identify over 150 fatty acids and 1,500 bacterial species in as little as 15 minutes. For microbial ID, no Gram stain or biochemical cards are needed and consumables cost as little as $3 USD per test.

The Sherlock™ MIS software includes many powerful tools, including: PLFA Analysis (PLFA Tools) Library Generation (LGS), Principal Component Analysis (PCA), and Electronic Records & Signatures (ERS).

For research use only.  Not for use in diagnostic procedures.

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Sherlock™ Mycobacteria ID System (MYCO-LCS)

Cellular mycolic acid analysis by High Performance Liquid Chromatography (HPLC) has been used for more than 20 years as a rapid and easy-to-use method for routine mycobacterial ID.

The Sherlock™ MYCO-LCS
is the only commercially-available mycobacterial ID system that uses mycolic
acid analysis by HPLC for
Mycobacterium tuberculosis ID.

The Sherlock™ MYCO-LCS identifies over 25 species
of mycobacteria and 35 species of non-mycobacteria, including: Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella.

Sherlock™ MYCO-LCS can separate M. bovis BCG from the TB complex and
M. scrofulaceum
from MAI.

The Sherlock™ MYCO-LCS uses the same procedure to identify all mycobacterial and non-mycobacterial species in less than 30 minutes from pure culture.  The cost per test for consumables is less than $6 USD.

For research use only.  Not for use in diagnostic procedures.

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Upcoming MIDI Events

October 07-08, 2015

Supply Side West

Booth # 3572
Mandalay Bay
Las Vegas, NV, USA

Booth # 105E25

Event Website


Soils, Oils & Forensics

The Sherlock software platform has expanded to solutions beyond species-level ID, which include the forensics analysis of fire debris residues (complex hydrocarbons patterns), edible oils with complex FAME patterns (e.g. fish oil, those with 18:1 isomers and cis/trans), and soil microbial community
analysis (PLFAs).

Sherlock X: Fire Debris Analysis

Edible Oil & Fish Oil Analysis

Soil Microbial Community Analysis

MIDI Product Brochure

A complete overview of MIDI's products and services.

MIDI Product Brochure

Please contact

for more information on our products and services.