Rapid & Accurate
Microbial Identification

MIDI Inc., located in Newark, Delaware, USA, has been developing rapid microbial identification solutions for agriculture, environmental and clinical laboratories since 1991.

MIDI manufactures three microbial identification solutions: The Sherlock® Microbial Identification System, Sherlock® Mycobacteria Identification System and Sherlock® DNA Sequencing Software.

In addition, we provide microbial identification contract lab services by DNA sequencing and Fatty Acid Analysis through our sister company, MIDI Labs.

MIDI is a Premier Channel Partner of Agilent Technologies and is a small veteran-owned business.

The Sherlock® Microbial Identification System (MIS)

Cellular fatty acid analysis by Gas Chromatography (FAME) has been used for more than 50 years as a rapid and
easy-to-use method for routine microbial ID.

The Sherlock® MIS is the
only commercially-available microbial ID system that uses FAME technology.  The system is U.S. CDC cleared for aerobic bacterial ID and
AOAC INTERNATIONAL cleared for Bacillus anthracis ID.

The Sherlock® MIS can identify over 1,500 bacterial and yeast species in as little as 15 minutes from pure culture, with no Gram stain or biochemical cards required.  Consumables cost as little as $3.00 USD per test.

The Sherlock® MIS software includes many powerful tools, including: custom library generation (LGS), principal component analysis (PCA), dendrogram, strain tracking and Electronic Records & Signatures (ERS).

Many Sherlock® MIS users opt to use the system to identify phospholipid fatty acids (PLFA), because of the precision of the system to name complex fatty acids.

For research use only.  Not for use in diagnostic procedures.

The Sherlock® Mycobacteria Identification System (MYCO-LCS)

Cellular mycolic acid analysis by High Performance Liquid Chromatography (HPLC) has been used for more than 15 years as a rapid and easy-to-use method for routine mycobacterial ID.

The Sherlock® MYCO-LCS
is the only commercially-available mycobacterial ID system that uses mycolic
acid analysis by HPLC for
Mycobacterium tuberculosis ID.

The Sherlock® MYCO-LCS identifies over 25 species
of mycobacteria and 35 species of non-mycobacteria, including: Corynebacteria, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella.

Sherlock® MYCO-LCS can separate M. bovis BCG from the TB complex and
M. scrofulaceum from MAI.

The Sherlock® MYCO-LCS uses the same procedure to identify all mycobacterial and non-mycobacterial species in less than 30 minutes from pure culture.  The cost per test is less than $6.00 USD
in consumables.

For research use only.  Not for use in diagnostic procedures.

UPCOMING PRODUCTS

Sherlock 6.2 Software

Windows 7 Compliance, updated libraries and Agilent 7890 GC Support

Sherlock Phospholipid Fatty
Acid (PLFA) Peak Naming Table & Method

Contains all PLFA biomarkers from Actinomycetes, Aerobes, Anaerobes & Fungi

Sherlock Edible Oil Peak Naming Table & Method

Contains fatty acids from all classes of vegetable oils, as well as Omega-3 fatty acids

MIDI Product Brochure

A complete overview of MIDI's products and services.

Please contact



for more information.