The Sherlock Mycobacteria Identification System is
composed of a Windows® 2000-based computer loaded with the MIDI Sherlock
software and an Agilent ChemStation (Version A.06.03 or above). The
computer is interfaced with an Agilent 1100/1200 HPLC.
Many of the hardware components of the MIDI System are designed to meet
specifications required for robust and reproducible performance. The
quaternary pump employs low pressure mixing to maintain a constant rate of
solvent delivery. The autosampler, with flow-through design, eliminates
carry over contamination and the Peltier temperature control is capable of
maintaining column temperatures to within 0.1C. A long-life
chromatographic column is able to provide consistent performance over
thousands of runs.
In addition, the MIDI system employs fluorescence detection, which
enhances sensitivity ca. 1000-fold compared with UV detection.
