The Sherlock Microbial Identification System (MIS) was launched in 1991 and represents a rapid, accurate and inexpensive solution for the identification of over 1,500 microbial species, fatty acids and PLFAs by GC-FAME analysis. The Sherlock MIS is recognized by the U.S. CDC for the identification of aerobic bacteria and is AOAC INTERNATIONAL cleared for the identification of Bacillus anthracis, the anthrax pathogen. The Sherlock MIS was created by microbiologists for microbiologists, so no GC experience is necessary.
In 2007 MIDI released Sherlock Instant FAMETM (I-FAME), a base-catalyzed GC-FAME extraction and analysis method. I-FAME is most useful for Gram-positive environmental aerobes and FAME analysis of non-microbes (e.g. animal tissue, edible oils, biofuel feedstocks, etc.). The turnaround time (TAT) for IFAME is less than
In 2011 MIDI released Sherlock Q-FAMETM (Q-FAME), an acid-catalyzed GC-FAME extraction and analysis method. Q-FAME is most useful for Aerobic bacteria of importance to pharmaceutical QC (including all USP 61/62 strains), as well as clinical reference laboratories. The turnaround time (TAT) for Q-FAME is less than 25 minutes.
What makes the Sherlock MIS unique is that it can be used for both fatty acid analysis (e.g. soil PLFAs, edible oils) and microbial identification. The system is U.S. FDA 21CFR Part 11 compliant and IQ/OQ packages are available to aid with system validation.
Key Features of the Sherlock MIS include:
- Fatty acid analysis (FAME, PLFA) and Microbial ID.
- Automated analysis and naming.
- Inexpensive per sample cost.
- Throughput over 100 samples per day.
- No biochemical kits or cards (microbial ID).
- Polyphasic Analysis with Sherlock DNA (microbial ID)
- Proven reliability and warranty.
Contact MIDI for further information on the Sherlock MIS System.